Journal:

Article Title: PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp.

doi: 10.1128/AAC.47.3.1125-1128.2003

Figure Lengend Snippet: PCR-restriction fragment length polymorphism patterns obtained after digestion with BsaI and BceAI. The restriction products were analyzed by electrophoresis on a 10% polyacrylamide gel stained with ethidium bromide. Wild-type C. jejuni reference strain NCTC 11168 and strains 00072, 01206, and 00039, with mutations A2075G, A2074C, and both A2074C and A2075G occurring in domain V of the 23S rRNA gene, were used. Lanes: M, 25-bp DNA Step Ladder molecular size markers (Promega); 1, nondigested PCR products of wild-type C. jejuni strain NCTC 11168; 2 to 5, BsaI-digested PCR products of the wild-type and A2075G, A2074C, and A2074C-A2075G mutant C. jejuni strains, respectively; 6 to 9, BceAI-digested PCR products of the wild-type, A2075G, A2074C, and A2074C-A2075G C. jejuni strains, respectively.

Article Snippet: Lanes: M, 25-bp DNA Step Ladder molecular size markers (Promega); 1, nondigested PCR products of wild-type C. jejuni strain NCTC 11168; 2 to 5, Bsa I-digested PCR products of the wild-type and A2075G, A2074C, and A2074C-A2075G mutant C. jejuni strains, respectively; 6 to 9, Bce AI-digested PCR products of the wild-type, A2075G, A2074C, and A2074C-A2075G C. jejuni strains, respectively.

Techniques: Electrophoresis, Staining, Mutagenesis